Single Molecule Mechano-Memory

Single molecule FRET reveals pore size and opening mechanism of a mechano-sensitive ion channel

The mechanosensitive channel of large conductance, which serves as a model system for mechanosensitive channels, has previously been crystallized in the closed form, but not in the open form. Ensemble measurements and electrophysiological sieving experiments show that the open-diameter of the channel pore is >25 Å, but the exact size and whether the conformational change follows a helix-tilt or...

ARTICLES ON SINGLE-MOLECULE PHYSICS AND CHEMISTRY Direct measurements of memory effects in single-molecule kinetics

Statistics and correlations of single-molecule sequences of modulated reactions are explicitly evaluated in the stochastic rate representation. The memory function, introduced through the Gaussian approximation of the stochastic rate expression, characterizes the correlation in single-molecule rate processes in a formalism similar to the stochastic line shape theory. Within this formalism, the ...

Memory and Spectral Diffusion in Single-Molecule Emission

Single Molecule Conformational Memory Extraction: P5ab RNA Hairpin

Extracting kinetic models from single molecule data is an important route to mechanistic insight in biophysics, chemistry, and biology. Data collected from force spectroscopy can probe discrete hops of a single molecule between different conformational states. Model extraction from such data is a challenging inverse problem because single molecule data are noisy and rich in structure. Standard ...

Extracting conformational memory from single-molecule kinetic data.

Single-molecule data often come in the form of stochastic time trajectories. A key question is how to extract an underlying kinetic model from the data. A traditional approach is to assume some discrete state model, that is, a model topology, and to assume that transitions between states are Markovian. The transition rates are then selected according to which ones best fit the data. However, in...